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Studies on the Binding of Iron to Transferrin and Conalbumin

Philip Aisen 1, Adela Leibman 2, and Haskell A. Reich 2

From the 1 From the Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, and the IBM Watson Laboratory at Columbia University, New York, New York 10025
2 From the IBM Watson Laboratory at Columbia University, New York, New York 10025

Electrophoretic studies of the binding of iron to transferrin indicate that the stability constants of the two binding sites are approximately equal. The iron in each site also acts independently in relaxing the protons of the solvent water. It appears likely, then, that the binding sites are equivalent and independent. Similar results have been obtained for conalbumin, corroborating the evidence that the two proteins have similar structure.

Submitted on September 30, 1965


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