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Mitochondrial Swelling and Lipid Peroxidation Studies with Mixtures of Thyroxine and Micromolar Concentrations of Certain Metal Ions

William D. Cash 1, Martin Gardy 1, Harold E. Carlson 1, and Enobong A. Ekong 1

From the 1 From the Department of Biochemistry, Cornell University Medical College, New York, New York 10021

Studies with isolated rat liver mitochondria were conducted in order to determine the influence of thyroxine on swelling and peroxidation of mitochondrial lipids caused by certain divalent metal ions. The effects of micromolar concentrations of certain divalent metal ions on the swelling action of thyroxine were also investigated.

Thyroxine blocks mitochondrial swelling and lipid peroxidation caused by Fe++. The hormone also blocks Cu++-induced swelling.

Swelling is strikingly potentiated when thyroxine and micromolar concentrations of Ca++ or Zn++ are tested in the presence of each other.

Cu++ and Sr++ at concentrations of 10 µm or less block the swelling action of thyroxine. Fe++ also blocks thyroxine swelling at relatively low concentrations of metal ion and hormone, but higher concentrations of the two agents produce swelling.

A strong lipid antioxidant action is exhibited by thyroxine at a concentration of 1 µm. This concentration of the hormone is about one-tenth that necessary to produce appreciable mitochondrial swelling.

The finding that the response to an organic swelling agent can be altered markedly by levels of metal ions that might be present adventitiously further illustrates the importance of considering metal contaminants in studies with isolated mitochondria.

Submitted on December 1, 1965


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