JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Holt, C. E.
Right arrow Articles by Herbert, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Holt, C. E.
Right arrow Articles by Herbert, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Turnover of Terminal Nucleotides of Soluble Ribonucleic Acid in Intact Reticulocytes

Charles E. Holt 1, Peteranne B. Joel 1, and Edward Herbert 1

From the 1 From the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

The incorporation of adenine-8-14C and cytidine-3H into the soluble ribonucleic acid (sRNA) of intact rabbit reticulocytes in vitro was studied. The 14C which is incorporated into the sRNA is located primarily in the adenosine of the terminal pCpCpA group. The incorporated 3H appears to be predominantly in the 2 cytidine monophosphate residues of the terminal group.

Studies on the kinetics of the incorporation of adenine and cytidine into ATP, CTP, and sRNA indicate a turnover time of 4 to 8 hours for the terminal adenosine and an average of about 30 hours for the 2 CMP residues of the terminal group.

Puromycin at 100 µg per ml had little or no effect on the incorporation of adenine and cytidine into the sRNA of the intact reticulocytes although it inhibited the rate of incorporation of leucine into protein by 98%.

Submitted on October 18, 1965


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1966 by the American Society for Biochemistry and Molecular Biology.