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The Enzymatic Reduction of Nicotinamide N-Oxide

From the ¹ From the Department of Biochemistry and Biophysics, University of California, Davis, California 95616

An enzymatic system which reduces nicotinamide N-oxide to nicotinamide was isolated from hog liver and purified about 280-fold. The reduction is dependent on the presence of reduced diphosphopyridine nucleotide or a low molecular weight constituent found in boiled liver supernatant. The two cofactors appear to be involved in the expression of two distinct enzymatic activities. Evidence has been presented which indicates both activities may be associated with a single protein or enzymatic unit. The DPNH-dependent reaction is the following.

H⁺ + DPNH + nicotinamide N-oxide DPN⁺ + nicotinamide + H₂O

The nature of the supernatant-dependent reaction is not completely understood, since the identity of the compound which is oxidized in the course of reduction of the N-oxide is not known.

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