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Polyribosomes and Protein Synthesis in the Spleen

From the ¹ From the National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014

Protein synthesis in rat spleens has been found to occur on polyribosomes. These structures, aggregates of two, three, four, or more ribosomes, become labeled after 1 min of incubation with radioactive amino acids. Incorporation occurs into nascent polypeptide chains which can be released by puromycin or "chased off" by ¹²C-amino acids. These polyribosomes stimulate cell-free protein synthesis in vitro in the presence of spleen or liver "pH 5 fraction," an energy-generating system, and an optimal magnesium concentration. Synthesis is inhibited by puromycin and by ribonuclease, which dissociates polyribosomes into monomers. Rat spleen polyribosomes thus have properties similar to those of ribosomal aggregates studied in other biological systems.

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