HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kwan, S.-W. Articles by Webb, T. E. Search for Related Content PubMed PubMed Citation Articles by Kwan, S.-W. Articles by Webb, T. E. Social Bookmarking                      What's this?

A Study of the Mechanism of Polyribosome Breakdown Induced in Regenerating Liver by 8-Azaguanine

From the ¹ From the McGill University Cancer Research Unit, McIntyre Medical Sciences Building, Montreal 2, Province of Quebec, Canada

Following the administration of 8-azaguanine to rats, a portion of the polyribosomes in regenerating liver are converted to monomers and dimers, with a concomitant inhibition of protein synthesis. Polyribosome breakdown induced by suboptimal doses of the base analogue and the incorporation of 8-azaguanine-¹⁴C into RNA are enhanced by pretreating the rats with low doses of 4-amino-5-imidazolecarboxamide. The dimers are labile, and break down to monomers after a short incubation at 37°. The monomer and dimer pool appears to be nonfunctional in vivo; only the residual polyribosomes are pulse labeled with l-leucine-¹⁴C. The latter finding suggests that the former species did not originate from breakdown of the latter species during isolation. The residual polyribosomes incorporated l-leucine-¹⁴C in vivo at rates comparable to those of polyribosomes in controls. The data are consistent with the incorporation of 8-azaguanine into certain species of RNA, probably messenger RNA, which are essential to the integrity of the polyribosome.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				I. B. Levitan and T. E. Webb Posttranscriptional Control in the Steroid-Mediated Induction of Hepatic Tyrosine Transaminase Science, January 16, 1970; 167(3916): 283 - 285. [Abstract] [PDF]