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Chemical Studies of Histone Acetylation

THE OCCURRENCE OF -N-ACETYLLYSINE IN THE f2a1 HISTONE

From the ¹ From The Rockefeller University, New York, New York 10021

The f2a1 histone was isolated from calf thymus nuclei which had been incubated in the presence of sodium acetate-1-¹⁴C. Following digestion of the histone with trypsin and Pronase, the peptides and amino acids were fractionated by exclusion chromatography and ion exchange chromatography. All of the radioactivity of the ¹⁴C-acetate-labeled f2a1 histone was recovered in one peak which was identified as -N-acetyllysine.

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