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Kinetic Properties of Rabbit Testicular Lactate Dehydrogenase Isozyme

From the ¹ From the Cátedra de Química Biológica, Facultades de Medicina y Odontología, Universidad Nacional de Córdoba, Córdoba, Argentina

The additional lactate dehydrogenase isozyme from rabbit testis and sperm, lactate dehydrogenase isozyme-1 (B₄) from heart, and lactate dehydrogenase isozyme-5 (A₄) from liver have been separated and partially purified by preparative starch gel electrophoresis.

Studies performed on these fractions comprised K_m and optimum substrate concentration for pyruvate, -ketobutyrate, and lactate, inhibition by substrate and product, effect of oxamate, oxalate, urea, some citric acid cycle metabolites, pH, and heating.

Results indicate that rabbit testicular lactate dehydrogenase isozyme is a distinct molecular form with peculiar catalytic properties. It showed different behavior against high substrate concentrations whether the direct or the reverse reaction was studied: striking inhibition by pyruvate, no effect with lactate. There is a remarkable correlation between these properties and metabolic requirements of mature spermatozoa.

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