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Ribonucleic Acid Synthesis in Isolated Mitochondria from Tetrahymena

From the ¹ From the Joseph Leidy Laboratory, Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104

Some conditions of RNA synthesis in vitro in isolated mitochondria of Tetrahymena pyriformis were investigated. Sucrose gradient centrifugation analyses and methylated albumin-coated Kieselguhr column chromatography of the RNA synthesized in vitro demonstrate that RNA ranging from 14 S to 18 S, not transfer RNA, was synthesized. Furthermore, the RNA is hybridizable with mitochondrial DNA, and the hybridization is not inhibited by the addition of RNA isolated from the material sedimenting at 105,000 x g after centrifugation of mitochondrial lysate for 1 to 2 hours. Although net transfer RNA synthesis does not occur, the transfer RNA fraction was labeled if ³H-ATP was used for incorporation, suggesting that the incorporation is due to the addition of ³H-ATP at the terminus of the transfer RNA molecule.

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