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From the
1 From the Department of Internal Medicine, The University of Texas Southwestern Medical School at Dallas, Dallas, Texas 75235
The intranuclear binding of radioactive hormone in prostate after the intravenous administration of testosterone-1,2-3H to rats has been studied. Nuclei obtained by sucrose density gradient centrifugation were extracted with buffer containing 0.6 m NaCl, and the soluble radioactivity was separated into bound and free fractions by gel filtration on Sephadex G-25 or G-200. As early as 15 min after testosterone administration, the radioactivity recovered in the bound form was predominantly dihydrotestosterone. By gel filtration of nuclear extracts on Sephadex G-200 and by the use of digestive enzymes, it was shown that dihydrotestosterone was bound to an acidic nuclear protein. Finally, several characteristics of this binding phenomenon were defined; the binding was stable to freezing for as long as 8 days, stable to short term incubation at 20° but not at 37°, and partially stable to repeated gel filtration on Sephadex.
The Intranuclear Binding of Testosterone and 5
-Androstan-17ß-ol-3-one by Rat Prostate
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