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Biosynthesis of Branched Chain Deoxysugars

IV. ISOLATION OF CYTIDINE DIPHOSPHATE 6-DEOXY-3-C-METHYL-2-O-METHYL-4-O-(O-METHYLGLYCOLYL)-l-ALDOHEXOPYRANOSIDE FROM AZOTOBACTER VINELANDII

From the ¹ From the Department of General Education and the Department of Chemistry, Faculty of Science, Nagoya University, Chikusa, Nagoya, Japan

A new cytidine nucleotide was found in Azotobacter vinelandii strain O. On the basis of chemical analyses, mass spectra, infrared spectra, nuclear magnetic resonance spectra, periodate oxidation studies, and a series of degradation studies followed by comparison with authentic samples, it is proposed that the new compound is cytidine-5'-diphospho-6-deoxy-3-C-methyl-2-O-methyl-4-O-(O-methylglycolyl)-l-aldohexopyranose. The compound is therefore an O-methylglycolic acid ester of cytidine-5'-diphospho-vinelose previously found in the same microorganism.

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