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The N,O-Diacetylmuramidase of Chalaropsis Species

II. PHYSICAL PROPERTIES

From the ¹ From the Department of Microbiology, Vanderbilt University School of Medicine, Nashville, Tennessee 37203

Lysozyme Chalaropsis is a single polypeptide chain with an apparent weight average molecular weight of 19,100 ± 900. The ultraviolet absorption is typical for proteins with an E^1%_{1 cm} = 24.8 ± 0.4, and its isoelectric point is weakly alkaline at 7.53. The sedimentation constant (s⁰_20,w) of 2.27 ± 0.14 exhibits no apparent concentration dependence, a phenomenon consistent with a symmetrical sedimenting mass. The secondary structure, as judged by optical rotatory dispersion in the ultraviolet, exhibits significant structural differences as compared to hen egg white lysozyme.

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