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The Nucleotide Sequence of Ribosomal 5 S Ribonucleic Acid from KB Cells

From the ¹ From the Metabolism Branch, National Cancer Institute, United States Department of Health, Education and Welfare, Public Health Service, National Institutes of Health, Bethesda, Maryland, 20014, and the Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510

Over 40 different large oligonucleotide fragments were obtained by partial digestion with taka-diastase ribonuclease T₁ or spleen acid ribonuclease of ³²P-labeled ribosomal 5 S RNA derived from KB cells (human epidermoid carcinoma line). The fragments were isolated by anion exchange column chromatography, further separated and purified by a second anion exchange column or by electrophoresis, and characterized after complete digestion with pancreatic ribonuclease or ribonuclease T₁ by a one- or two-dimensional electrophoresis fractionation procedure. From information provided by these analyses and previous reports on oligonucleotide sequences of KB cell ribosomal 5 S RNA we derived the total nucleotide sequence of KB cell 5 S RNA. Some homology exists between the two halves of the KB cell 5 S RNA nucleotide sequence and also between the sequence of KB cell 5 S RNA and that of Escherichia coli 5 S RNA. The possible secondary structure of KB cell 5 S RNA is discussed in view of the partial splitting patterns of the RNA with ribonuclease T₁.

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