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Isolation and Composition of a Photosynthetic Reaction Center Complex from Rhodopseudomonas spheroides

From the ¹ From the Section of Genetics, Development and Physiology, Cornell University, Ithaca, New York 14850

A photosynthetic reaction center complex has been isolated from the blue-green mutant strain R-26 of Rhodopseudomonas spheroides by Triton X-100 treatment, sucrose density gradient centrifugation, and gel filtration. In this complex, the only bacteriochlorophyll is the specialized reaction center component, P870, and the associated P800. The reaction center preparations appear nearly homogeneous by gel filtration and polyacrylamide gel disc electrophoresis. Gel filtration indicates a particle weight near 6.5 x 10⁵, and the particle contains approximately 35% lipid-extractable material. Other components of the complex and their approximate ratios are: 1 P870, 2 P800, 13 ubiquinone, 2 cytochrome b₅₆₂, 1 cytochrome c₅₅₂, 16 total iron, and 9 copper. The light-induced absorbance changes of this complex are indistinguishable from those which have been associated with the primary photochemical reactions of bacterial photosynthesis.

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