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Biosynthesis of Cellulose in Vitro from Guanosine Diphosphate d-Glucose with Enzymic Preparations from Phaseolus aureus and Lupinus albus

From the ¹ From the Department of Biochemistry, University of California, Berkeley, California 94720

The incorporation of d-glucose-¹⁴C from guanosine diphosphate d-glucose-¹⁴C into a ß-1,4-linked polymer (cellulose) is catalyzed by a particulate preparation from Phaseolus aureus and Lupinus albus. The particulate enzyme preparations from both plants can be solubilized by extraction with digitonin; the solubilized proteins are also active in synthesizing ß-(1 4)-glucan as the original particulate preparations. The reaction reaches half the maximal incorporation in less than 1 min.

Upon degradation of the polymer, ß-(1 4)-radioactive cellodextrins are formed. However, a small proportion of mannose-¹⁴C is also obtained, indicating the presence of an epimerase which interconverts d-glucose and d-mannose. A glycolipid, is, apparently, not involved as an intermediate in the biosynthesis of the ß-(1 4)-glucan from GDP-d-glucose.

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