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Turnover of Cytochromes Labeled with -Aminolevulinic Acid-³H in Rat Liver

From the ¹ From the Departments of Medicine and Biochemistry, Pritzker School of Medicine, The University of Chicago and Argonne Cancer Research Hospital, Chicago, Illinois 60637

Rat liver hemeproteins are isotopically labeled after injection of -aminolevulinic acid-³H, a specific precursor of heme. Incorporation of isotope is restricted to the prosthetic group of hemeproteins, and cytochromes with high specific radioactivity are generated. Subsequent measurements of biological decay in vivo can be made, and permit calculation of half-lives for several cytochromes. Cytochromes b and c are associated with the inner mitochondrial membrane and have similar half-lives, 5.5 and 6.1 days. Cytochrome b₅ is associated with the outer mitochondrial membrane, and its half-life is 4.4 days. Microsomal cytochrome b₅ is renewed with a half-life of 2.3 days. Labeling the protein moiety of microsomal cytochrome b₅ with arginine-¹⁴C (guanido) yielded a half-life of 2.5 days.

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