| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
From the
1 From the Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37203
1. Livers from normal and alloxan diabetic rats were perfused in vitro with a medium in which the concentration of free fatty acid was maintained at relatively constant levels by the infusion of a complex of oleic acid and bovine serum albumin. Concentrations of free fatty acid up to 3.5 mm in the cell-free perfusate were attained. 2. Throughout the range of concentration of free fatty acid which might be expected in the blood under normal and pathological conditions in vivo, the uptake of free fatty acid by the liver in vitro was proportional to the concentration in the medium; rates of uptake of free fatty acid approached saturation only at concentrations of free fatty acid in excess of those encountered in vivo. 3. The accumulation of triglyceride in livers from normal and most probably also in livers from alloxan diabetic rats was proportional to the concentration of free fatty acid in the medium. In contrast to triglyceride, the concentrations of total phospholipid, cholesterol, cholesteryl esters, and free fatty acid in the livers from normal and alloxan diabetic animals was independent of the concentration of free fatty acid in the perfusate. 4. Ketogenesis by livers from normal rats was dependent on the concentration of oleate in the medium; the maximal rates of production of ketone bodies were observed at 1.7 mm oleate. The rate of ketogenesis by livers from normal rats never equaled that by livers from alloxan diabetic animals, regardless of the concentration of free fatty acid in the medium. Although ketogenesis by livers from diabetic rats was responsive to the presence of oleate in the medium, similar and rapid rates of production of ketone bodies were observed over the entire range of concentration of free fatty acid. When livers from normal animals were exposed to high concentrations of oleate and the free fatty acid was withdrawn subsequently, the rates of ketogenesis diminished rapidly even though the concentration of triglyceride was elevated in the liver; glucagon, infused for 3 hours after oleate had been discontinued, could sustain the high rate of ketogenesis only for the short period during which free fatty acid remained in the medium. These observations force us to consider that, unlike free fatty acid, triglyceride fatty acid which accumulates in the livers of normal, fed rats is oxidized slowly, and that glucagon may not exert a major ketogenic action by stimulation of a hepatic triglyceride lipase.
Hepatic Lipid Metabolism in Experimental Diabetes
V. THE EFFECT OF CONCENTRATION OF OLEATE ON METABOLISM OF TRIGLYCERIDES AND ON KETOGENESIS
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. R. Miller, P. Siripurkpong, J. Hawes, A. Majdalawieh, H.-S. Ro, and R. S. McLeod The trans-10, cis-12 isomer of conjugated linoleic acid decreases adiponectin assembly by PPAR{gamma}-dependent and PPAR{gamma}-independent mechanisms J. Lipid Res., March 1, 2008; 49(3): 550 - 562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang, R. M. Lewis, C. Wang, N. Hales, and C. D. Byrne Maternal dietary iron restriction modulates hepatic lipid metabolism in the fetuses Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2005; 288(1): R104 - R111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tiikkainen, R. Bergholm, S. Vehkavaara, A. Rissanen, A.-M. Hakkinen, M. Tamminen, K. Teramo, and H. Yki-Jarvinen Effects of Identical Weight Loss on Body Composition and Features of Insulin Resistance in Obese Women With High and Low Liver Fat Content Diabetes, March 1, 2003; 52(3): 701 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Linden, A. Sjoberg, L. Asp, L. Carlsson, and J. Oscarsson Direct effects of growth hormone on production and secretion of apolipoprotein B from rat hepatocytes Am J Physiol Endocrinol Metab, December 1, 2000; 279(6): E1335 - E1346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. V. Chirieac, L. R. Chirieac, J. P. Corsetti, J. Cianci, C. E. Sparks, and J. D. Sparks Glucose-stimulated insulin secretion suppresses hepatic triglyceride-rich lipoprotein and apoB production Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1003 - E1011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Taghibiglou, D. Rudy, S. C. Van Iderstine, A. Aiton, D. Cavallo, R. Cheung, and K. Adeli Intracellular mechanisms regulating apoB-containing lipoprotein assembly and secretion in primary hamster hepatocytes J. Lipid Res., March 1, 2000; 41(4): 499 - 513. [Abstract] [Full Text]
|
|
|
|
 |
|
 I. Rusyn, C. A. Bradham, L. Cohn, R. Schoonhoven, J. A. Swenberg, D. A. Brenner, and R. G. Thurman Corn oil rapidly activates nuclear factor-{kappa}B in hepatic Kupffer cells by oxidant-dependent mechanisms Carcinogenesis, November 1, 1999; 20(11): 2095 - 2100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-M. Hebbachi, A.-M. Brown, and G. F. Gibbons Suppression of cytosolic triacylglycerol recruitment for very low density lipoprotein assembly by inactivation of microsomal triglyceride transfer protein results in a delayed removal of apoB-48 and apoB-100 from microsomal and Golgi membranes of primary rat hepatocytes J. Lipid Res., October 1, 1999; 40(10): 1758 - 1768. [Abstract] [Full Text]
|
|
|
|
 |
|
 Z. Zhang, K. Cianflone, and A. D. Sniderman Role of Cholesterol Ester Mass in Regulation of Secretion of ApoB100 Lipoprotein Particles by Hamster Hepatocytes and Effects of Statins on That Relationship Arterioscler. Thromb. Vasc. Biol., March 1, 1999; 19(3): 743 - 752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-M. Brown, J. Castle, A.-M. Hebbachi, and G. F. Gibbons Administration of n-3 Fatty Acids in the Diets of Rats or Directly to Hepatocyte Cultures Results in Different Effects on Hepatocellular ApoB Metabolism and Secretion Arterioscler. Thromb. Vasc. Biol., January 1, 1999; 19(1): 106 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Murata, K. Ishihara, and H. Saito Hepatic Fatty Acid Oxidation Enzyme Activities Are Stimulated in Rats Fed the Brown Seaweed, Undaria pinnatifida (Wakame) J. Nutr., January 1, 1999; 129(1): 146 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang, D. G. Hole, T. H. M. Da Costa, and R. D. Evans Alterations in myocardial lipid metabolism during lactation in the rat Am J Physiol Endocrinol Metab, August 1, 1998; 275(2): E265 - E271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Yancey and W. G. Jerome Lysosomal sequestration of free and esterified cholesterol from oxidized low density lipoprotein in macrophages of different species J. Lipid Res., July 1, 1998; 39(7): 1349 - 1361. [Abstract] [Full Text]
|
|
|
|
|
|
P. J. Coussons, C. S. Bourgeois, D. Wiggins, and G. F. Gibbons Selective Recruitment of ApoB-48 for the Assembly of VLDL in Rat Triacylglycerol-Enriched Hepatocytes Arterioscler. Thromb. Vasc. Biol., July 1, 1996; 16(7): 889 - 897. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
