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The Participation of an Enzyme-bound Oxygen Group in a Coenzyme A Transferase Reaction

From the ¹ From the Molecular Biology Institute, and the Department of Chemistry, UCLA, Los Angeles, California 90024

A well supported mechanism for succinyl-CoA:3-ketoacid coenzyme A transferase (EC 2.8.3.5) involving an enzyme-CoA intermediate requires the participation of an oxygen atom of the enzyme or of the CoA moiety. Measurements of loss of ¹⁸O from acetoacetate show that CoA oxygens do not participate. An oxygen-containing group on the enzyme is reversibly labeled from succinate-¹⁸O in the presence of acetoacetyl-CoA or succinyl-CoA. The ¹⁸O-labeled enzyme does not contain succinyl or CoA moieties. A binding of acetoacetate-¹⁴C to the enzyme preparation does not appear to reflect an anhydride precursor to the enzyme-CoA form.

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