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Isolation and Characterization of Bovine Thyrocalcitonin

From the ¹ From the Molecular Disease Branch, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014
² From the Departments of Biochemistry and Pharmacology, Armour Pharmaceutical Company, Kankakee, Illinois 60901

Bovine thyrocalcitonin has been isolated in homogeneous form following a 40,000-fold purification. The isolated hormone contains 32 amino acids including Arg₁, His₁, Thr₂, Ser₄, Glu₁, Pro₂, Gly₃, Ala₁, Val₁, Met₁, Asn₃, Asp₁, Lys₁, Tyr₂, Phe₂, Leu₃, Trp₁, Cys₂. The purified peptide is resolved by chromatography on cellulose thin layer plates or carboxymethyl cellulose into two components. These two components differ in the oxidative state of the methionine residue; however, both forms have an equal biological activity of 200 Medical Research Council units per mg. The amino acid composition of the bovine hormone differs from the porcine by 3 residues, the human by 18, and salmon by 16.

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