HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by van den Hamer, C. J. A. Articles by Ashwell, G. Search for Related Content PubMed PubMed Citation Articles by van den Hamer, C. J. A. Articles by Ashwell, G. Social Bookmarking                      What's this?

Physical and Chemical Studies on Ceruloplasmin

IX. THE ROLE OF GALACTOSYL RESIDUES IN THE CLEARANCE OF CERULOPLASMIN FROM THE CIRCULATION

From the ¹ From the Department of Medicine, Albert Einstein College of Medicine, New York, New York 10461
² From the National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014

The survival time in plasma of incompletely desialylated ceruloplasmin, labeled with ⁶⁴Cu or with tritium, was studied in the rat. Removal of less than 20% of the terminal sialic acid, with consequent exposure of the penultimate galactosyl residues, resulted in the rapid disappearance from the circulation of more than 50% of the injected dose. Statistical analysis of the experimental results by means of the Poisson distribution equation supports the estimate that the exposure of no more than 2 galactosyl residues is sufficient to effect prompt clearance of ceruloplasmin from the circulation. Examination of the specificity of the two neuraminidase preparations used in this study revealed that each of the enzymes possessed a preferential affinity for different sialic acid residues, although the results from each were statistically predictable. Consequently, the removal of any 2 sialic acid residues from 1 molecule of ceruloplasmin appears to be incompatible with the continued survival of that molecule in the circulation.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. J.S. Jones, D. I. Papac, E. H. Chin, R. Keck, S. A. Baughman, Y. S. Lin, J. Kneer, and J. E. Battersby Selective clearance of glycoforms of a complex glycoprotein pharmaceutical caused by terminal N-acetylglucosamine is similar in humans and cynomolgus monkeys Glycobiology, May 1, 2007; 17(5): 529 - 540. [Abstract] [Full Text] [PDF]

				E. I. Park, Y. Mi, C. Unverzagt, H.-J. Gabius, and J. U. Baenziger The asialoglycoprotein receptor clears glycoconjugates terminating with sialic acid{alpha}2,6GalNAc PNAS, November 22, 2005; 102(47): 17125 - 17129. [Abstract] [Full Text] [PDF]

				E. I. Park and J. U. Baenziger Closely Related Mammals Have Distinct Asialoglycoprotein Receptor Carbohydrate Specificities J. Biol. Chem., September 24, 2004; 279(39): 40954 - 40959. [Abstract] [Full Text] [PDF]

				E. I. Park, S. M. Manzella, and J. U. Baenziger Rapid Clearance of Sialylated Glycoproteins by the Asialoglycoprotein Receptor J. Biol. Chem., February 7, 2003; 278(7): 4597 - 4602. [Abstract] [Full Text] [PDF]

				R. J. Stockert, A. G. Morell, and I. H. Scheinberg Mammalian Hepatic Lectin Science, October 25, 1974; 186(4161): 365 - 366. [Abstract] [PDF]