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Amino Acid Sequence of Human Chains

I. TRYPTIC PEPTIDES OF PROTEIN Ha

From the ¹ From the Department of Zoology, Indiana University, Bloomington, Indiana 47401

For determination of the amino acid sequence of the human Bence-Jones Protein Ha, the protein was reduced and aminoethylated and digested with trypsin. Twenty-two tryptic peptides were isolated by chromatography. The complete sequence was determined for 11 peptides including all but one of those comprising the variable NH₂-terminal half of the protein. The remaining peptides were subjected only to amino acid and end group analysis. Altogether, the the position of 119 out of the 217 residues in the protein was determined. None of the tryptic peptides in the NH₂-terminal half of the molecule was identical in sequence with those reported for other human chains, whereas all of the tryptic peptides covering the sequence from position 109 through the COOH terminus corresponded exactly to the sequence earlier reported for the constant part of human chains.

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