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Molar Extinction Coefficient and Iron and Sulfide Content of Clostridial Ferredoxin

From the ¹ From the Department of Biochemistry, University of California, Berkeley, California 94720

The molar extinction coefficient at 390 nm of Clostridium acidi-urici ferredoxin was determined by three independent methods: dry weight determination, amino acid analysis, and amino acid release by carboxypeptidase A treatment. The value of ₃₉₀ based on the latter method is 30,600 m^-1 cm^-1. The content of iron was determined by atomic absorption spectrophotometry and the content of sulfide was determined from the amount of ³⁵S-sulfide incorporated into ferredoxin in the reconstitution from apoferredoxin. Ferredoxin was found to contain 8 moles of both iron and sulfide.

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