Consequences of Expression of the "Relaxed" Genotype of the RC Gene
LIPID SYNTHESIS
Burton E. Tropp 1, Linda C. Meade 1, and Paul J. Thomas 1
From the
1 From the Division of Science and Engineering, Richmond College of The City University of New York, Staten Island, New York 10301, and the Mayo Foundation, Rochester, Minnesota 55901
Two pairs of strains that differ in the rel-1 locus were compared for their ability to synthesize protein, RNA, and lipid in the presence and absence of a required amino acid. In the absence of a required amino acid, protein synthesis ceased in both relaxed control (RCrel) and stringent control (RCstr) cells, and RNA synthesis ceased only in the RCstr cells. Lipid synthesis continued at an approximately equal rate in the presence or absence of a required amino acid in both RCrel and RCstr cells. Analysis of the lipid extracts showed a decrease in cardiolipin in RCrel cells deprived of a required amino acid. Otherwise, the distribution of the lipids was not affected by the absence of a required amino acid in either the RCrel or RCstr cells and was quite similar in both types of cells. The fatty acid distribution also appeared unaffected. These results are consistent with the view that the control of RNA synthesis exercised by the rel-1 locus cannot be simply at the level of nucleoside triphosphate availability.
Submitted on September 5, 1969
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