HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Jabbal, I. Articles by Schachter, H. Search for Related Content PubMed PubMed Citation Articles by Jabbal, I. Articles by Schachter, H. Social Bookmarking                      What's this?

Pork Liver Guanosine Diphosphate-l-Fucose Glycoprotein Fucosyltransferases

From the ¹ From the Department of Biochemistry, University of Toronto, Toronto 5, Canada

Two fucosyltransferases have been found in pork liver which transfer l-fucose from GDP-l-fucose into various derivatives of human plasma ₁-acid glycoprotein. One of these enzymes incorporates l-fucose into the terminal positions of ₁-acid glycoprotein prosthetic groups and into the disaccharide, galactosyl-(ß,1 4)-N-acetylglucosamine. This enzyme does not transfer fucose to lactose, galactosyl(ß,1 3)-N-acetylglucosamine, galactosyl-(ß,1 6)-N-acetylglucosamine, and the - and ß-methyl-d-galactopyranosides. The substrate specificity indicates that the fucosyltransferase is responsible for the incorporation of l-fucose into plasma glycoproteins in vivo. The enzyme is membrane-bound and requires Triton X-100 and divalent cation for optimum activity. The other pork liver fucosyltransferase incorporates l-fucose into a position on the oligosaccharide prosthetic group of ₁-acid glycoprotein at which l-fucose does not occur in the native glycoprotein; the function of this enzyme in pork liver is not known.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?