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Nicotinic Acid Metabolism

VIII. TRACER STUDIES ON THE INTERMEDIARY ROLES OF -METHYLENEGLUTARATE, METHYLITACONATE, DIMETHYLMALEATE, AND PYRUVATE

From the ¹ From the Laboratory of Biochemistry, Section on Enzymes, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014

Extracts of nicotinate-grown Clostridum barkeri ferment [7-¹⁴C]nicotinate to ¹⁴CO₂ and a mixture of volatile and non-volatile acidic products. This conversion is markedly stimulated by the addition of certain intermediates of the over-all fermentation pathway, namely pyruvate, -methyleneglutarate, methylitaconate, or dimethylmaleate. In large scale experiments [¹⁴C]-methyleneglutarate, [¹⁴C]methylitaconate, and [¹⁴C]dimethylmaleate were detected among the products of [7-¹⁴C]nicotinate metabolism. When specifically labeled -methyleneglutarate and dimethylmaleate were fermented, the distribution of ¹⁴C among the final products (acetate, propionate, and carbon dioxide) was as predicted from the earlier tracer experiments. The carboxyl carbon of pyruvate is the most likely precursor of carbon dioxide in the fermentation.

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