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Tryptophan Synthetase ß2 Subunit

PRIMARY STRUCTURE OF THE PYRIDOXYL PEPTIDE FROM THE PSEUDOMONAS PUTIDA ENZYME

Richard Maurer 1 and I. P. Crawford 1

From the 1 From the Department of Microbiology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Two labeled peptides were found in tryptic digests of NaB3H4-reduced, performic acid-oxidized Pseudomonas putida ß2 subunit. These two pyridoxyl peptides were identical except for the presence or absence of an NH2-terminal arginyl residue. The tryptic peptides which comprise the two halves of the pyridoxal phosphate-binding segment were isolated from digests of nonreduced, performic acid-oxidized protein. The primary structure deduced for this region of the protein is: [see PDF for sequence].

Submitted on April 9, 1971


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