HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gospodarowicz, D. Search for Related Content PubMed PubMed Citation Articles by Gospodarowicz, D. Social Bookmarking                      What's this?

Single Step Purification of Ovine Luteinizing Hormone by Affinity Chromatography

From the ¹ From The Salk Institute for Biological Studies, Post Office Box 1809, San Diego, California 92112

Affinity chromatography has been applied to the problem of specifically removing luteinizing hormone from sera used to supplement tissue culture media. Horse or fetal calf sera, passed through a column of an anti-luteinizing hormone immunoglobulin fraction linked covalently to agarose, were specifically depleted in their content of luteinizing hormone as seen by radioimmunoassay and by their effect on cultures of ovarian cells dependant on luteinizing hormone for their growth.

Affinity chromatography has also been applied to the one step isolation of luteinizing hormone from a partially purified or crude extract of ovine pituitary glands. Partially purified extracts or crude extracts of pituitary glands, passed through a column of an anti-luteinizing hormone immunoglobulin fraction linked covalently to agarose, were depleted in their luteinizing hormone content. Elution of the column by 6 m guanidine HCl pH 1.5 resulted in the recovery of the hormone which was further characterized by chemical and biological analysis as well as its electrophoretic pattern. It was demonstrated that affinity chromatography can be used to isolate preparations of highly homogeneous ovine luteinizing hormone in one step.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. Starkey, S Cohen, and D. Orth Epidermal growth factor: identification of a new hormone in human urine Science, September 5, 1975; 189(4205): 800 - 802. [Abstract] [PDF]