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Fractionation of the C-Apoproteins from Human Plasma Very Low Density Lipoproteins

ARTIFACTUAL POLYMORPHISM FROM CARBAMYLATION IN UREA-CONTAINING SOLUTIONS

From the ¹ From the Molecular Disease Branch, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014

The use of urea in gel and ion exchange chromatography is shown to facilitate greatly the fractionation of the C-apolipoproteins from human plasma very low density lipoproteins. However, it is demonstrated that the use of urea at room temperature can lead to the production of artifactual polymorphism through carbamylation of the C-apolipoproteins. The bead column technique of Sachs and Painter has been adapted for use with superfine Sephadex gels and urea solutions for the rapid and reproducible fractionation of the very low density apolipoproteins.

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