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Pyridine Nucleotide Metabolism in Escherichia coli

II. NIACIN STARVATION

From the ¹ From the Department of Biology, University of Utah, Salt Lake City, Utah 84112, and the Department of Biochemistry, College of Medicine, University of the Philippines, Manila, Philippines

The effect of niacin starvation has been studied in a niacin-requiring auxotroph of Escherichia coli. If a culture is totally deprived of niacin, cells continue to divide until the total pyridine nucleotide content has fallen from 1.9 x 10⁶ to 1.2 x 10⁵ molecules per cell. During starvation, the relative proportion of the pyridine nucleotides changes greatly: the TPN: DPN ratio increases from 0.30 to over 2.0 and nicotinic acid mononucleotide accumulates until it is present at concentrations comparable to DPN. The changes in the distribution of the pyridine nucleotides suggest that DPN is turning over during niacin starvation and that the normally observed breakdown of TPN to DPN during exponential growth is inhibited. If cells are starved for niacin by balanced growth under limiting concentrations of niacin, less disproportion in the TPN: DPN ratio is observed, and growth occurs with a pyridine nucleotide content as low as 4 x 10⁴ molecules per cell.

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