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Aminotripeptidase of Swine Kidney

II. AMINO ACID COMPOSITION AND MOLECULAR WEIGHT

Dennis Chenoweth 1, Douglas M. Brown 1, Maria Antonieta Valenzuela 1, and Emil L. Smith 1

From the 1 From the Department of Biological Chemistry, UCLA School of Medicine, and the Molecular Biology Institute, University of California, Los Angeles, California 90024

Three different forms of aminotripeptidase have been obtained from swine kidney. As studied in the ultracentrifuge by sedimentation-equilibrium, these forms are: 1. a stable monomer of 71,100 molecular weight that consists of a single polypeptide chain lacking disulfide bridges; 2. a stable dimer that contains two apparently identical subunits of the same size as the monomer; and 2'. an unstable dimer that dissociates spontaneously to the monomeric units during sedimentation-equilibrium studies at 20°. Aminotripeptidases-1 and -2 have the same amino acid composition.

Submitted on October 10, 1972


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