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Synthesis and Nucleolytic Degradation of Uracil-containing Deoxyribonucleic Acid by Escherichia coli Deoxyribonucleic Acid Polymerase I

From the ¹ From the Department of Biochemistry, College of Biological Sciences, University of Minnesota, St. Paul, Minnesota 55101

The incorporation of uracil into DNA in an in vitro repair reaction catalyzed by native Escherichia coli DNA polymerase I renders the newly synthesized uracil-containing portion of the resultant polymer susceptible to an endonucleolytic-like cleavage by the polymerase itself. This response is related to the presence of the 5' 3' nuclease activity of this enzyme, since subtilisin-treated polymerase which lacks this nuclease activity also fails to attack uracil-containing DNA in this way.

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