JBC, Vol. 250, Issue 10, 3996-4006, May, 1975
Biosynthesis of chondroitin sulfate proteoglycan. Subcellular distribution of glycosyltransferases in embryonic chick brain
A. E. Brandt, J. J. Distler and G. W. Jourdian
The subcellular distributions of five glycoslytransferases involved in the
biosynthesis of the chondroitin sulfate proteoglycan and of a sixth
glycosyltransferase, presumably involved in glycoprotein biosynthesis, were
examined in 13-day chick enbryo brain. Fractionation studies performed by
the procedure of Gray and Whittaker (Gray, E.G., and Whittaker, V.P. (1962)
J. Anat. (London) 96, 79-88) revealed that three of the six enzymes were
directly associated with the membrane fraction of synaptosome-enriched
preparations; varying amounts of the remaining glycosyltransferases were
distributed between the 100,000 times g supernatant and the
synaptosome-enriched fraction after differential and density gradient
centrifugation of crude chick brain homogenates. The time of appearance of
three of the glycosyltransferases was examined in chick embryo brain tissue
at several stages of development. The brain content of each
glycosyltransferase increased rapidly between day 7 and hatching at day 21.
A sharp decline in each of the glycosyltransferase activities occurred at
hatching.
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