HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Sweadner, K. J. Articles by Goldin, S. M. Search for Related Content PubMed PubMed Citation Articles by Sweadner, K. J. Articles by Goldin, S. M. Social Bookmarking                      What's this?

JBC, Vol. 250, Issue 10, 4022-4024, May, 1975

Reconstitution of active ion transport by the sodium and potassium ion-stimulated adenosine triphosphatase from canine brain

K. J. Sweadner and S. M. Goldin

Sodium and potassium ion-stimulated adenosine triphosphatase ((Na+ + K+)-ATPase) was partially purified from canine brain gray matter and reconstituted into vesicles of phosphatidylcholine. A proportion of the enzyme molecules was reconstituted into sealed vesicles with the ATP-hydrolyzing site facing the outside of the vesicles. ATP was added to the outside of the vesicles after they had equilibrated with radioactive tracer, and the resulting active transport of Na+ and K+ was followed. Unlike the purified kidney renal medulla enzyme used in an earlier study, the brain enzyme transports both Na+ and K+(Rb+). Vesicles were made in solutions with different proportions of NaCl and KCl, and over the range studied, an average of 1.8 Rb+ ions were transported for every 3 Na+ ions. When ATP is depleted, the transported ions diffuse back to their equilibrium level in the vesicles.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?