JBC, Vol. 250, Issue 17, 7074-7077, Sep, 1975
Thermal regulation of the membrane lipid composition of Escherichia coli. Evidence for the direct control of fatty acid synthesis
J. E. Cronan Jr
Starvation of strains of Escherichia coli which are glycerol auxotrophs and
are also defective in beta oxidation results in the accumulation of large
amounts of free fatty acid (Cronan, J. E., Jr., Weisberg, L. W., and Allen,
R. G. (1975) J. Biol. Chem. 250, 5835-5840). We now report that the ratio
of saturated to unsaturated species appearing in the free fatty acid
fraction depends on the incubation temperature at the time of synthesis of
these acids. This result indicates that fatty acid synthesis is one site of
the thermal control of phospholipid fatty acid composition. We also report
experiments on the incorporation of exogenously supplied fatty acids into
membrane phospholipids. The effect of temperature on this incorporation
supports the hypothesis that a second site of thermal regulation acts at
the level of phosphatidic acid synthesis.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
