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JBC, Vol. 250, Issue 8, 2823-2829, Apr, 1975

The ultraviolet endonuclease of bacteriophage T4. Further characterization

K. Minton, M. Durphy, R. Taylor and E. C. Friedberg

The T4 ultraviolet endonuclease was previously shown to produce strand incisions (nicks) in ultraviolet-irradiated DNA on the 5' side of thymine dimers. The present studies demonstrate that the purified endonuclease creates 3'-OH and 5'-P termini at the sites of nicking. Photoreactivation of ultraviolet-sensitive sites, thereby demonstrating directly endonucleause has a molecular weight of approximately 18,000 and attacks ultraviolet-irradiated single-stranded Escherichia coli and M-13 DNA.
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