JBC, Vol. 251, Issue 3, 609-623, Feb, 1976
Total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer RNA from Escherichia coli. 5. Synthesis of the deoxyribopolynucleotide segments representing the nucleotide sequence 71-103
E. Jay, P. J. Cashion, M. Fridkin, B. Ramamoorthy, K. L. Agarwal, M. H. Caruthers and H. G. Khorana
Chemical syntheses of the pentadecanucleotide,
d(G-G-T-G-G-G-G-T-T-C-C-C-G-A-G), the undecanucleotides,
d(G-G-T-G-G-G-G-T-T-C-C) and d(C-C-C-C-A-C-C-A-C-G-G), the decanucleotide,
d(G-T-A-A-T-G-C-T-T-T), and the nonanucleotides, d(A-T-T-A-C-C-C-G-T) and
d(A-G-T-A-A-A-A-G-C) are described. The deoxyribopolynucleotides together
represent the DNA duplex corresponding to the nucleotide sequence 71-103
(from the 3'-end) of the gene for the tyrosine suppressor tRNA. Synthesis
of the guanine-rich undecanucleotide d(G-G-T-G-G-G-G-T-T-C-C) was performed
by the use of a new protecting group for the guanine ring, the
methylbutyryl group. The heptanucleotide
d[(MeOTr)mbG-mbG-T-mbG-mbG-mbG-mbG], prepared by the new method, was
condensed with the tetranucleotide d[panC-anC-T-T(Ac)]. All of the
condensations described followed previously developed chemical principles
and started with the N- and 5'-protected deoxyribonucleosides. Successive
condensations at the 3'-end with protected mononucleotides, preformed di-,
tri-, or tetranucleotides gave products which were separated by anion
exchange chromatography and characterized by chemical and enzymatic
methods.
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