JBC, Vol. 251, Issue 9, 2680-2687, May, 1976
Structure and function of beta-blucosidases in Dictyostelium discoideum
R. L. Dimond and W. F. Loomis
There are two isozymes of beta-glucosidase in developing cells of
Dictyostelium discoideum. A procedure for screening large numbers of clones
for beta-glucosidase activity was utilized to obtain mutations which
directly affect the activity. We recovered seven strains which lack both
isozymes and four strains with residual activity in which enzymatic and
physical properties of both isozymes are altered. Beta-Glucosidase appears
to act as a block to selfing in macrocyst formation as shown by the fact
that ssite mating type to form macrocyst-like structures. Immunological
evidence utilizing antisera prepared against purified beta-glucosidase-1
demonstrates that most of the glycosidases in Dictyostelium discoideum
share a common antigenic determinant which appears to be added
post-translationally. The two isozymes of beta-glucosidase share common
protein subunits but the antigenic determinant is either lacking or masked
in beta-glucosidase-2. This may account for some of the enzymatic and
physical differences between the two isozymes.
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