JBC, Vol. 252, Issue 10, 3379-3381, May, 1977
Effects of chaotropic agents versus detergents on dihydroorotate dehydrogenase
H. J. Forman and J. Kennedy
As chaotropic salts are generally believed to affect water structure in a
manner which increases lipophilicity of water, they may seem to be capable
of substituting for detergents in the solubilization of particulate enzyme.
Although solubilization either by detergents or by chaotropic salts has
been demonstrated with several membrane proteins, the effects these agents
have on the properties and activity of an enzyme may be quite different.
This is illustrated by the effects on mammalian mitochondrial
dihydroorotate dehydrogenase. Stability of the solubilized enzymic activity
is dependnet on the presence of a detergent and maximum enzymic activity is
observed at the critical micelle concentration of the detergent. Addition
of low concentrations of various anions of the chaotropic series further
enhances activity while higher concentrations of these anions, although
increasing solubility of the enzyme, irreversibly inhibit catalysis.
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