JBC, Vol. 252, Issue 10, 3538-3547, May, 1977
Determination of the nucleotide sequence of part of the regulatory region for the galactose operon from Escherichia coli
J. Sklar, S. Weissman, R. E. Musso, R. DiLauro and B. de Crombrugghe
We have determined the sequence of 59 base pairs in the DNA preceding the
site for initiation of transcription in the galactose operon. DNA from a
lambdagal transducing phage was digested with restriction endonucleases to
obtain a DNA fragment from the gal regulatory region. This fragment extends
from 59 base pairs prior to the transcription initiation site through the
45 base pairs which specify the 5'-terminal sequence of gal mRNA. Analyses
of RNA transcripts derived from this fragment and a variety of direct DNA
sequence analyses allowed us to deduce the following sequence for the DNA
in this fragment: (formula: see text) Position +1 corresponds to the site
for initiation of gal mRNA synthesized in the presence of cyclic AMP and
its receptor protein, CRP. Transcription experiments indicate, however,
that this fragment lacks some element of the gal promoter required for the
stimulation of transcription by CRP-cAMP. There are, nonetheless, some
similarities between the gal sequence preceding the transcription start
site and the sequences of other promoter regions. These include the
heptamer sequence T-A-T-G-G-T-T (--12 to --8) and the sequence
A-C-A-C-T-T-T (--36 to --30).
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
