JBC, Vol. 252, Issue 10, 3561-3564, May, 1977
Reduced synthesis of [14C]mannosyl oligosaccharide-lipid by membranes prepared from concanavalin A-resistant Chinese hamster ovary cells
S. S. Krag, M. Cifone, P. W. Robbins and R. M. Baker
We compared the synthesis of mannosyl oligosaccharide-lipid by membranes of
wild type and concanavalin A-resistant Chinese hamster ovary cells during
incubations with GDP-[14C]mannose and UDP-N-acetylglucosamine. The
membranes prepared from exponentially growing mutant cells incorporated 10-
to 70-fold less [14C]mannose into oligosaccharide-lipid than did membranes
of wild type cells. The lectin-resistant cells are temperature-sensitive
for growth. Using temperature-resistant revertants, we showed that the
reduction in labeled oligosaccharide-lipid correlated with resistance to
concanavalin A rather than with the temperature-sensitive phenotype.
Tunicamycin, a specific inhibitor of oligosaccharide-lipid synthesis,
reduced the synthesis of the oligosaccharide-lipid by membranes of wild
type cells but did not affect the residual synthesis measured in membranes
of the mutant cells. As the mutant cells were grown to high density, there
was a marked increase in the synthesis of oligosaccharide-lipid by the
membranes.
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