HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pfeffer, S. R. Articles by Chamberlin, M. J. Search for Related Content PubMed PubMed Citation Articles by Pfeffer, S. R. Articles by Chamberlin, M. J. Social Bookmarking                      What's this?

JBC, Vol. 252, Issue 15, 5403-5407, Aug, 1977

Binding of Escherichia coli RNA polymerase to T7 DNA. Displacement of holoenzyme from promoter complexes by heparin

S. R. Pfeffer, S. J. Stahl and M. J. Chamberlin

Escherichia coli RNA polymerase holoenzyme bound to promoter sites on T7 DNA is attacked and inactivated by the polyanion heparin. The highly stable RNA polymerase-T7 DNA complex formed at the major T7 A1 promoter can be completely inactivated by treatment with heparin, as shown by monitoring the loss of activity of such complexes, and by gel electrophoresis of the RNA products transcribed. The rate of this inactivation is much faster than the rate of dissociation of RNA polymerase from promoter complexes, and thus represents a direct attack of heparin on the polymerase molecule bound at promoter A1. Experiments employing the nitrocellulose filter binding technique suggest that heparin inactivates E. coli RNA polymerase when bound to T7 DNA by directly displacing the enzyme from the DNA. RNA polymerase bound at a minor T7 promoter (promoter C) is much less sensitive to heparin attack than enzyme bound at promoter A1. Thus, the rate of inactivation of RNA polymerase-T7 DNA complexes by heparin is dependent upon the structure of the promoter involved even though the inhibitor binds to a site on the enzyme molecule.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. M. Gaines, N. L. Carty, F. Tiburzi, M. Davinic, P. Visca, J. A. Colmer-Hamood, and A. N. Hamood Regulation of the Pseudomonas aeruginosa toxA, regA and ptxR genes by the iron-starvation sigma factor PvdS under reduced levels of oxygen Microbiology, December 1, 2007; 153(12): 4219 - 4233. [Abstract] [Full Text] [PDF]

				H. Zhi, X. Wang, J. E. Cabrera, R. C. Johnson, and D. J. Jin Fis Stabilizes the Interaction between RNA Polymerase and the Ribosomal Promoter rrnB P1, Leading to Transcriptional Activation J. Biol. Chem., November 21, 2003; 278(47): 47340 - 47349. [Abstract] [Full Text] [PDF]

				M. Kamali-Moghaddam and E. P. Geiduschek Thermoirreversible and Thermoreversible Promoter Opening by Two Escherichia coli RNA Polymerase Holoenzymes J. Biol. Chem., August 8, 2003; 278(32): 29701 - 29709. [Abstract] [Full Text] [PDF]

				I. Artsimovitch, V. Svetlov, K. S. Murakami, and R. Landick Co-overexpression of Escherichia coli RNA Polymerase Subunits Allows Isolation and Analysis of Mutant Enzymes Lacking Lineage-specific Sequence Insertions J. Biol. Chem., March 28, 2003; 278(14): 12344 - 12355. [Abstract] [Full Text] [PDF]

				M. Cashel, L. M. Hsu, and V. J. Hernandez Changes in Conserved Region 3 of Escherichia colisigma 70 Reduce Abortive Transcription and Enhance Promoter Escape J. Biol. Chem., February 14, 2003; 278(8): 5539 - 5547. [Abstract] [Full Text] [PDF]

				J. Ederth, I. Artsimovitch, L. A. Isaksson, and R. Landick The Downstream DNA Jaw of Bacterial RNA Polymerase Facilitates Both Transcriptional Initiation and Pausing J. Biol. Chem., September 27, 2002; 277(40): 37456 - 37463. [Abstract] [Full Text] [PDF]

				M. Susa, R. Sen, and N. Shimamoto Generality of the Branched Pathway in Transcription Initiation by Escherichia coli RNA Polymerase J. Biol. Chem., May 3, 2002; 277(18): 15407 - 15412. [Abstract] [Full Text] [PDF]

				J. Xu, B. C. McCabe, and G. B. Koudelka Function-Based Selection and Characterization of Base-Pair Polymorphisms in a Promoter of Escherichia coli RNA Polymerase-{sigma}70 J. Bacteriol., May 1, 2001; 183(9): 2866 - 2873. [Abstract] [Full Text]

				R. Sen, H. Nagai, V. J. Hernandez, and N. Shimamoto Reduction in Abortive Transcription from the lambda PR Promoter by Mutations in Region 3 of the sigma 70 Subunit of Escherichia coli RNA Polymerase J. Biol. Chem., April 17, 1998; 273(16): 9872 - 9877. [Abstract] [Full Text] [PDF]

				Y. N. Zhou and D. J. Jin The rpoB mutants destabilizing initiation complexes at stringently controlled promoters behave like "stringent" RNA polymerases in Escherichia coli PNAS, March 17, 1998; 95(6): 2908 - 2913. [Abstract] [Full Text] [PDF]

				L. M. Heisler, G. Feng, D. J. Jin, C. A. Gross, and R. Landick Amino Acid Substitutions in the Two Largest Subunits of Escherichia coli RNA Polymerase That Suppress a Defective Rho Termination Factor Affect Different Parts of the Transcription Complex J. Biol. Chem., June 14, 1996; 271(24): 14572 - 14583. [Abstract] [Full Text] [PDF]

				S. Daube and P. von Hippel Functional transcription elongation complexes from synthetic RNA-DNA bubble duplexes Science, November 20, 1992; 258(5086): 1320 - 1324. [Abstract] [PDF]