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JBC, Vol. 252, Issue 19, 6841-6845, Oct, 1977
J. P. Thiery, R. Brackenbury, U. Rutishauser and G. M. Edelman
The aggregation of cells from dissociated neural retinas of chick embryos
can be inhibited by antibodies prepared against whole retinal cells. In
order to identify the antigens involved, substances released by retinal
tissues in culture were tested for their ability to neutralize specifically
the inhibition by antibody of cell adhesion. Using this assay, three active
polypeptides from the culture supernatant were purified 500-fold by gel
filtration and polyacrylamide gel electrophoresis. Rabbit antibodies
prepared against these purified supernatant activities inhibited cell
adhesion and reacted only with the three polypeptides. Immunoprecipitation
by the specific antibodies of 3H-labeled proteins from a detergent extract
of embryonic retinal cell membranes yielded a polypeptide having a Mr of
140,000 in sodium dodecyl sulfate. This precipitation was inhibited in the
presence of the three culture supernatant polypeptides that had activity,
suggesting that they contained antigenic determinants in common with the
140,000 Mr surface component. They therefore represent all or parts of this
cell surface molecule that were released into solution during tissue
culture. The data are consistent with the hypothesis that the 140,000 Mr
polypeptide is intimately involved in initial adhesion among neural cells.
Adhesion among neural cells of the chick embryo. II. Purification and characterization of a cell adhesion molecule from neural retina
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