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JBC, Vol. 252, Issue 2, 560-562, Jan, 1977
J. N. Livingston, P. A. Gurny and D. H. Lockwood
Significant amounts of H2O2 were produced when the polyamines, spermine, or
spermidine were incubated with a Krebs-Ringer phosphate buffer that
contained bovine serum albumin (Fraction V). This effect was specific for
certain amines and could be prevented by treatment of the albumin fraction
with isoniazid or aminoguanidine. These features suggest that H2O2 is
formed during oxidative deamination of the polyamines catalized by spermine
oxidase, a known contaminant of Fraction V bovine serum albumin. The
insulin-like effects elicited by polyamines in fat cells (e.g. enhancement
of glucose transport and inhibition of cAMP-mediated lipolysis) were
dependent on H2O2 production. Incubation of cells with catalase or
treatment of the albumin fraction with isoniazid abolished the stimulation
of glucose uptake by polyamines but did not alter the stimulatory effects
of insulin or vitamin K5. The H2O2 generating activity was partially
separated from the albumin by gel filtration; only those fractions which
formed H2O2 provided support for the activation of glucose transport by
polyamines. Also, the time needed to activate glucose uptake was markedly
shortened by incubation of the albumin buffer with the polyamines before
addition of the cells. These findings indicate that the polyamines do not
themselves mimic the actions of insulin but that the insulin-like effects
result from the formation of H2O2 which has been shown to stimulate glucose
transport.
Insulin-like effects of polyamines in fat cells. Mediation by H2O2 formation
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