JBC, Vol. 252, Issue 20, 7337-7343, Oct, 1977
Organization of ribosomal protein genes of Escherichia coli as analyzed by polar insertion mutations
S. R. Jaskunas and M. Nomura
Several mutants of lambdaspc1 and lambdafus3 have been isolated carrying
DNA insertion elements that were selected for their ability to reduce the
expression of the spc gene. The sizes and locations of the insertions on
the phage genomes were determined by heteroduplex analysis. They were found
to be located at different positions in the Spc transcription unit. The
effect of these insertions on the expression of the ribosomal protein genes
carried by these phages in ultraviolet light-irradiated bacteria was
investigated. The insertions at intermediate positions in the transcription
unit reduced the expression of some of the genes in the unit but not
others. Assuming that the genes whose expressions were reduced are distal
to the insertion, it was possible to determine the relative position of
most of the genes in the unit. The results indicate the order of genes in
the Spc transcription unit is: promoter, L14, L24, L5, S14, S8, L6, L18,
(S5, L15, L30).
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
