JBC, Vol. 252, Issue 22, 8182-8195, Nov, 1977
Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. VIII. Isolation and sequences of peptides from a tryptic hydrolysate of the maleylated protein
B. M. Austen, J. F. Nyc, D. M. Brown and E. L. Smith
The NAD-specific glutamate dehydrogenase of Neurospora crassa was
S-carboxymethylated with [14C]iodoacetate, maleylated, and hydrolyzed with
trypsin. The isolation and sequences of the resulting peptides are
described. These peptides gave information on the structure of the protein
that was previously unknown and gave many overlaps of previously isolated
segments of the protein.