| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
JBC, Vol. 252, Issue 3, 943-949, Feb, 1977
J. L. Gibson and F. R. Tabita
Ribulose-1,5-bisphosphate (Rbu-P2) carboxylase isolated from Rhodopseudomonas sphaeroides 2.4.1.Ga was separated into two different forms by DEAE-cellulose column chromatography. Both forms, designated Peak I and Peak II have been purified to homogeneity by the criterion of polyacrylamide disc-gel electrophoresis. The Peak I carboxylase has a molecular weight of 550,000, while the Peak II carboxylase is a smaller protein having a molecular weight of approximately 360,000. Sodium dodecyl sulfate electrophoresis revealed a large subunit for both enzymes which migrates similarly to the large subunit of spinach Rbu-P2 carboxylase. The Peak I enzyme also exhibited a small subunit having a molecular weight of 11,000. No evidence for a smaller polypeptide was found associated with the Peak II enzyme. Antisera prepared against the Peak I enzyme inhibited Peak I enzymatic activity, but had no effect on the activity of the Peak II enzyme. The two enzymes exhibited marked differences in catalytic properties. The Peak I enzyme exhibits optimal activity at pH 8.0 and is inhibited by low concentrations of 6-phosphogluconate, while the Peak II enzyme has a pH optimum of 7.2 and is relatively insensitive to 6-phosphogluconate.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Andersson Catalysis and regulation in Rubisco J. Exp. Bot., May 1, 2008; 59(7): 1555 - 1568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. R. Tabita, T. E. Hanson, H. Li, S. Satagopan, J. Singh, and S. Chan Function, Structure, and Evolution of the RubisCO-Like Proteins and Their RubisCO Homologs Microbiol. Mol. Biol. Rev., December 1, 2007; 71(4): 576 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Caldwell, M. R. MacLean, and P. R. Norris Ribulose bisphosphate carboxylase activity and a Calvin cycle gene cluster in Sulfobacillus species Microbiology, July 1, 2007; 153(7): 2231 - 2240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. P. Tourova, E. M. Spiridonova, I. A. Berg, B. B. Kuznetsov, and D. Yu. Sorokin Occurrence, phylogeny and evolution of ribulose-1,5-bisphosphate carboxylase/oxygenase genes in obligately chemolithoautotrophic sulfur-oxidizing bacteria of the genera Thiomicrospira and Thioalkalimicrobium Microbiology, July 1, 2006; 152(7): 2159 - 2169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Romagnoli and F. R. Tabita A Novel Three-Protein Two-Component System Provides a Regulatory Twist on an Established Circuit To Modulate Expression of the cbbI Region of Rhodopseudomonas palustris CGA010. J. Bacteriol., April 1, 2006; 188(8): 2780 - 2791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yoshizawa, K. Toyoda, H. Arai, M. Ishii, and Y. Igarashi CO2-Responsive Expression and Gene Organization of Three Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Enzymes and Carboxysomes in Hydrogenovibrio marinus Strain MH-110 J. Bacteriol., September 1, 2004; 186(17): 5685 - 5691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. van Keulen, A. N. J. A. Ridder, L. Dijkhuizen, and W. G. Meijer Analysis of DNA Binding and Transcriptional Activation by the LysR-Type Transcriptional Regulator CbbR of Xanthobacter flavus J. Bacteriol., February 15, 2003; 185(4): 1245 - 1252. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Elsaied and T. Naganuma Phylogenetic Diversity of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large-Subunit Genes from Deep-Sea Microorganisms Appl. Envir. Microbiol., April 1, 2001; 67(4): 1751 - 1765. [Abstract] [Full Text]
|
|
|
|
|
|
G. van Keulen, L. Dijkhuizen, and W. G. Meijer Effects of the Calvin Cycle on Nicotinamide Adenine Dinucleotide Concentrations and Redox Balances of Xanthobacter flavus J. Bacteriol., August 15, 2000; 182(16): 4637 - 4639. [Abstract] [Full Text]
|
|
|
|
|
|
K. M. Horken and F. R. Tabita The "Green" Form I Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase from the Nonsulfur Purple Bacterium Rhodobacter capsulatus J. Bacteriol., July 1, 1999; 181(13): 3935 - 3941. [Abstract] [Full Text]
|
|
|
|
|
|
J. M. Dubbs and F. R. Tabita Two Functionally Distinct Regions Upstream of the cbbI Operon of Rhodobacter sphaeroides Regulate Gene Expression J. Bacteriol., September 15, 1998; 180(18): 4903 - 4911. [Abstract] [Full Text]
|
|
|
|
|
|
S. H. Baker, S. Jin, H. C. Aldrich, G. T. Howard, and J. M. Shively Insertion Mutation of the Form I cbbL Gene Encoding Ribulose Bisphosphate Carboxylase/Oxygenase (RuBisCO) in Thiobacillus neapolitanus Results in Expression of Form II RuBisCO, Loss of Carboxysomes, and an Increased CO2 Requirement for Growth J. Bacteriol., August 15, 1998; 180(16): 4133 - 4139. [Abstract] [Full Text]
|
|
|
|
 |
|
 D Morse, P Salois, P Markovic, and J. Hastings A nuclear-encoded form II RuBisCO in dinoflagellates Science, June 16, 1995; 268(5217): 1622 - 1624. [Abstract] [PDF]
|
|
|
|
 |
|
 J. M. Dubbs, T. H. Bird, C. E. Bauer, and F. R. Tabita Interaction of CbbR and RegA* Transcription Regulators with the Rhodobacter sphaeroides cbbIPromoter-Operator Region J. Biol. Chem., June 16, 2000; 275(25): 19224 - 19230. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
