JBC Ideal method for primary cell transfection

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JBC, Vol. 252, Issue 5, 1728-1731, Mar, 1977

Turbidimetric ultracentrifugation. Application to the study of human serum very low density lipoprotein distributions

S. K. Ma, V. N. Schumaker and C. M. Knobler

In this communication it is shown that the sedimentation coefficient distribution may be accurately measured for very large particles using turbidimetric techniques and the ultraviolet-scanning analytical ultracentrifuge. A principal advantage is that turbidity is a function of the product of concentration and molecular weight; thus, large particles may be observed even when present in very small amounts. We propose to call this method of analysis "turbidimetric ultracentrifugation." We have used turbidimetric ultracentrifugation ot determine the sedimentation coefficient distribution for a sample of human serum very low density lipoproteins. This distribution is compared to that found with conventional schlieren techniques with good agreement.
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