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JBC, Vol. 252, Issue 6, 1917-1926, Mar, 1977
D. Y. Twumasi and I. E. Liener
A procedure is described for the purification of the elastase and
chymotrypsin-like enzymes from purulent sputum. This procedure permitted
the isolation of 132 mg and 120 mg of the elastase and chymotrypsin-like
enzymes, respectively, from 230 g of purulent sputum. The elastase enzymes
consist of a family of five isozymes, and at least three isozymes comprise
the chymotrypsin-like enzyme system. The elastases proved to be
immunologically identical with the corresponding enzyme of human
leukocytes. These enzymes were characterized with respect to molecular
weight, amino acid and carbohydrate composition, several kinetic
parameters, and inhibition by various synthetic and natural inhibitors. The
properties so found were comparable to those which had been previously
reported by others for the elastase and chymotrypsin-like enzymes isolated
directly from leukocytic granules.
Proteases from purulent sputum. Purification and properties of the elastase and chymotrypsin-like enzymes
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