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JBC, Vol. 252, Issue 8, 2670-2674, Apr, 1977

Adriamycin interactions with T4 DNA polymerase. Two modes of template-mediated inhibition

M. F. Goodman and G. M. Lee

We examined the effect of adriamycin on kinetics of DNA synthesis catalyzed by DNA polymerase purified from bacteriophage T4-infected Escherichia coli. Two distinct modes of enzyme inhibition occur: uncompetitive and competitive at "low" and "high" drug:DNA nucleotide molar ratios, respectively. Competitive inhibition is not observed unless an unblocked amino group is present on the sugar (daunosamine) moiety. A model is proposed to relate the enzyme inhibition kinetics to intercalative and ionic binding of adriamycin to DNA.
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