JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ljungquist, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ljungquist, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

JBC, Vol. 252, Issue 9, 2808-2814, May, 1977

A new endonuclease from Escherichia coli acting at apurinic sites in DNA

S. Ljungquist

A new DNA endonuclease has been purified 3000-fold from Escherichia coli. The enzyme specifically catalyzes the formation of single strand breaks at apurinic and apyrimidinic sites in DNA, but has no activity on intact or single-stranded DNA. Further, the enzyme shows little or no activity on heavily ultraviolet-irradiated DNA, but cleaves x-irradiated DNA, presumably at apurinic and apyrimidinic sites introduced by the radiation treatment. The enzyme, which is tentatively named endonuclease IV, has no detectable associated exonuclease or DNA N-glycosidase activity and does not seem to be identical with any previously known E. coli endonuclease. Endonuclease IV has no Mg2+ requirement, and is fully active in the presence of EDTA. Enzyme activity is stimulated by 0.2 to 0.3 M NaCl and is unusually salt-resistant. Further, the enzyme is fairly heat-stable, and is not inhibited by tRNA. The sidimentation coefficient, S(o)20,w, is 3.4 S. It seems that endonuclease IV is active in DNA repair.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
I. V. Kutyavin, D. Milesi, Y. Belousov, M. Podyminogin, A. Vorobiev, V. Gorn, E. A. Lukhtanov, N. M. J. Vermeulen, and W. Mahoney
A novel endonuclease IV post-PCR genotyping system
Nucleic Acids Res., November 14, 2006; 34(19): e128 - e128.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. K. Bhakat, S. K. Mokkapati, I. Boldogh, T. K. Hazra, and S. Mitra
Acetylation of Human 8-Oxoguanine-DNA Glycosylase by p300 and Its Role in 8-Oxoguanine Repair In Vivo.
Mol. Cell. Biol., March 1, 2006; 26(5): 1654 - 1665.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. A. Ishchenko, E. Deprez, A. Maksimenko, J.-C. Brochon, P. Tauc, and M. K. Saparbaev
Uncoupling of the base excision and nucleotide incision repair pathways reveals their respective biological roles
PNAS, February 21, 2006; 103(8): 2564 - 2569.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. M. Muller, S. C. Stebel, S. Knall, G. Zipf, H. S. Bernauer, and K. M. Arndt
Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolution
Nucleic Acids Res., August 1, 2005; 33(13): e117 - e117.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. G. Georgakilas, P. V. Bennett, D. M. Wilson III, and B. M. Sutherland
Processing of bistranded abasic DNA clusters in {gamma}-irradiated human hematopoietic cells
Nucleic Acids Res., October 19, 2004; 32(18): 5609 - 5620.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Faure, J.-F. Constant, P. Dumy, and M. Saparbaev
2'-Deoxyribonolactone lesion produces G->A transitions in Escherichia coli
Nucleic Acids Res., May 24, 2004; 32(9): 2937 - 2946.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. A. Ishchenko, G. Sanz, C. V. Privezentzev, A. V. Maksimenko, and M. Saparbaev
Characterisation of new substrate specificities of Escherichia coli and Saccharomyces cerevisiae AP endonucleases
Nucleic Acids Res., November 1, 2003; 31(21): 6344 - 6353.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. M. Kerins, R. Collins, and T. V. McCarthy
Characterization of an Endonuclease IV 3'-5' Exonuclease Activity
J. Biol. Chem., January 24, 2003; 278(5): 3048 - 3054.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
B. J. Haas, M. Sandigursky, J. A. Tainer, W. A. Franklin, and R. P. Cunningham
Purification and Characterization of Thermotoga maritima Endonuclease IV, a Thermostable Apurinic/Apyrimidinic Endonuclease and 3'-Repair Diesterase
J. Bacteriol., May 1, 1999; 181(9): 2834 - 2839.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. Sandigursky, A. Yacoub, M. R. Kelley, W. A. Deutsch, and W. A. Franklin
The Drosophila Ribosomal Protein S3 Contains a DNA Deoxyribophosphodiesterase (dRpase) Activity
J. Biol. Chem., July 11, 1997; 272(28): 17480 - 17484.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1977 by the American Society for Biochemistry and Molecular Biology.